Giemsa C-banding and silver-staining for cytological studies in Pisum
نویسندگان
چکیده
منابع مشابه
Fluorescence and Giemsa Banding Techniques
The C group chromosomes in man cannot be distinguished solely on morphological grounds, thus, the nature of an extra C chromosome remains unknown unless it is an X chromosome, which can be identified by its late DNA-replication pattern. Extra autosomes belonging to the C group have been observed with or without mosaicism in a wide variety of blood disorders (cf, de la Chapelle et al, 1970; Hell...
متن کاملGiemsa C-banding and the evolution of wheat.
The somatic chromosomes of common wheat, Triticum aestivum L. (2n = 6x = 42), and those of two of its diploid progenitors and T. speltoides, have been individually identified by a Giemsa staining technique. In wheat, telocentric chromosomes were used to aid the recognition of individual chromosomes, and an ideogram has been constructed depicting the C-band positions. There is no similarity in t...
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Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...
متن کاملan acetocarmine staining procedure for chromosome banding studies of immature pollen in triticeae
studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. chromosome staining of immature and adult pollen grains through conven-tional acetocarmine procedure is tedious and often leads to unsatisfactory results. an acetocarmine stain, used in conjugation with carnoy’s solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike b...
متن کاملA silver-Schiff reaction for cytological studies: light microscope observations
A method is presented in which a complex metal-ion (ammoniacal silver) is substituted for leuco-basic fuchsin in the well-known Schiff reaction for aldehydes. The reagent is reduced to metallic silver by tissue aldehydes and submicroscopic particles of the metal are deposited at the reactive sites. When the reagent is used in the Feulgen reaction chromosomes may be 'stained' in a specific manne...
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ژورنال
عنوان ژورنال: Hereditas
سال: 2009
ISSN: 0018-0661
DOI: 10.1111/j.1601-5223.1981.tb01730.x